Under which condition would a typical human enzyme most likely lose its shape and stop working?

Core Concept

Human enzymes are tuned to body temperature, roughly 37 degrees Celsius. At 80 degrees, thermal motion shakes the chain violently enough to snap the weak hydrogen bonds, ionic contacts, and hydrophobic packing that maintain the native fold, and the protein often aggregates irreversibly. Near-neutral pH and balanced salt preserve the very interactions that high heat destroys.