The uncoupler DNP shuttles protons across the inner mitochondrial membrane, partially collapsing the proton gradient. Which outcome follows?

Core Concept

Chemiosmotic coupling ties ATP synthesis to the proton-motive force stored across the inner mitochondrial membrane. An uncoupler offers protons an alternate return path, so the gradient drains without driving ATP synthase. Electron flow and oxygen uptake persist, and the freed energy is released as heat, which explains the raised metabolic rate and body temperature seen with uncouplers. ATP made per NADH drops because much of the conserved energy is dissipated rather than captured.