Unit 2: Cell Structure and Function

AP Biology: 159 practice questions with detailed explanations.

Unit Study Guide

Executive Summary

Unit 2 tours the cell's compartments and explains how membranes control what enters and leaves. Compartmentalization is the theme: separate spaces run separate chemistry.

Subcellular components

Eukaryotes hold membrane-bound organelles: nucleus (DNA), rough ER (protein synthesis), smooth ER (lipid synthesis, detox), Golgi (packaging), mitochondria (ATP), chloroplasts (sugars), lysosomes (digestion), vacuoles (storage). Ribosomes — not membrane-bound — assemble proteins in all cells.

Surface area to volume

As cells grow, volume (r³) outpaces surface area (r²), so exchange across the membrane becomes limiting. Small cells and folded membranes (cristae, villi, root hairs) raise SA:V.

Membrane structure

The fluid mosaic model: a phospholipid bilayer with embedded proteins and cholesterol. Hydrophilic heads face water; hydrophobic tails face inward. Small nonpolar molecules cross freely; ions and large polar solutes need channels or carriers.

Transport

Passive transport runs down gradients without energy: diffusion, facilitated diffusion (channel and carrier proteins), osmosis. Active transport pumps solutes against gradients using ATP (Na⁺/K⁺ pump). Endocytosis and exocytosis move large cargo in vesicles.

Tonicity

Water moves toward higher solute concentration. Hypertonic surroundings shrink animal cells (crenation) and plasmolyze plant cells; hypotonic surroundings swell animal cells (lysis) but turgid plant cells are protected by cell walls. Isotonic = no net flow.

Compartmentalization

Separate organelles allow incompatible reactions to coexist (lysosome's acid hydrolases vs cytosol). The endosymbiotic theory explains mitochondria and chloroplasts: double membranes, own DNA, bacterial-like ribosomes.

Top 5 Concepts to Master

  1. 1Predict movement direction from concentration gradients for each transport mode.
  2. 2Decide cell fate in hyper/hypo/isotonic solutions for animal vs plant cells.
  3. 3Relate surface area to volume to cell size and folded membranes.
  4. 4Map protein traffic: rough ER → Golgi → vesicles → membrane.
  5. 5Use the endosymbiotic theory to explain mitochondrial and chloroplast features.

Key Terms & Definitions

Practice with Flashcards
Phospholipid bilayer

Two-layer membrane with hydrophilic heads out, hydrophobic tails in.

Fluid mosaic model

Membrane of moving phospholipids with embedded proteins.

Selective permeability

Membranes let some substances cross but not others.

Diffusion

Net movement of particles down their concentration gradient.

Facilitated diffusion

Passive transport through a channel or carrier protein.

Osmosis

Diffusion of water across a semipermeable membrane.

Hypertonic

Higher solute concentration outside; water leaves the cell.

Hypotonic

Lower solute concentration outside; water enters the cell.

Isotonic

Equal solute concentrations; no net water movement.

Active transport

Protein-mediated pumping against a gradient using ATP.

Endocytosis

Vesicle-mediated uptake of material into a cell.

Exocytosis

Vesicle-mediated release of material from a cell.

Surface area to volume ratio

Sets exchange capacity; decreases as cells enlarge.

Endosymbiotic theory

Mitochondria/chloroplasts descend from engulfed prokaryotes.

Common Misconceptions: Exam Traps

Facilitated diffusion uses ATP.

Correct: It is passive — channels and carriers only speed movement down an existing gradient.

Water moves toward lower solute concentration.

Correct: Water moves toward the higher solute concentration (lower water potential).

Plant cells burst in hypotonic solutions like animal cells.

Correct: The cell wall resists swelling; plant cells become turgid, which is healthy.

All proteins can cross the membrane freely.

Correct: Most proteins are far too large and polar; they move by vesicle trafficking.

The nucleus is the only organelle with DNA.

Correct: Mitochondria and chloroplasts carry their own small circular genomes.

Question Bank Breakdown

By difficulty

easy 72medium 66hard 21

By topic

Cell Structure: Subcellular Components 59Cell Structure and Function 43Mechanisms of Transport 29Membrane Transport 23Compartmentalization 23Tonicity and Osmoregulation 21Membrane Permeability 19Facilitated Diffusion 9Cell Size 7Origins of Cell Compartmentalization 7

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